AcceGen

  Since its discovery in 1993,   microRNA (miRNA)  has proven its value in biotechnological research many times over. MicroRNA is a small (approximately 22 nucleotides), endogenous, non-coding RNA molecule that occurs in animals, plants, and certain viruses. These molecules have been found to play a hugely important role in the regulation of gene expression after transcription, and well over one thousand miRNAs can be found in the human genome. They are believed to influence at least 30 percent of human genes.     MiRNA inhibitors and mimics promote RNA silencing by cleaving the messenger RNA (mRNA) strand into separate pieces, destabilizing the mRNA via shortening of the poly(A) tail, and/or interfering with the translation of mRNA into proteins.   MiRNA agomir/antagomir synthetic molecules provide biotech researchers with a powerful tool for their investigations.   About Agomir Agomir is a chemically modified double-strand miRNA mimic that regulates ...

  Stable cell lines  are an important research tool used by pharmaceutical companies, for gene therapy research, and for compounding research. Each stable cell line is constructed using various methods to determine whether it will be a knockout cell line or an overexpression cell line.     The process uses do vary but, in general, stable cell lines are constructed by integrating the  exogenous plasmid DNA  into a host cell  chromosome  so that the host cell can express the protein of interest for a longer period. The exogenous is capable of being stably expressed in cell division for as long as two weeks.   What Is the Benefit of Using Stable Cell Lines? Using stable cell lines helps improve screen processes to determine the appropriate number of cells required for various compounds and pharmaceutical drugs. In addition, stable cell lines can help reduce costs since the use of excessive conversion of dyes and virus packaging can be greatly re...