AcceGen

Endothelial cells (ECs) cover the lumen of blood vessels. Human umbilical vein endothelial cells (HUVECs) are a relatively simple and available human EC type, suitable for large scale preparation [1]. Thus, HUVECs are widely used  in vitro  model for ECs to study vascular endothelium properties and the main biological pathways involved in endothelium function. Isolation and culture of HUVECs    The primary isolated  Human Umbilical Vein Endothelial Cells  are the most popular ECs used in a number of researches probably because human umbilical veins are relatively more available than other types of blood vessels. Generally, primary HUVECs need to be isolated from human umbilical vein vascular wall by a collagenase treatment, then inoculated on coated culture flask and incubated in a 37 °C-5% CO2 incubator by standard protocol with a relatively minimal equipment. Cell confluency can be monitored by phase-contrast microscopy [1]. Of course, the prima...

Human Primary Cells Human primary cells  are those that have been obtained directly from the healthy or pathological  in vivo  tissue of a human subject through a mechanical or enzymatic process. They are distinct from  animal primary cells , which are commonly taken from rats, mice, dogs, horses, or pigs; and  immortalized cell lines , which, through spontaneous mutation or deliberate modification, can be passaged  in vitro  indefinitely. These primary cells are widely used in biochemistry research to study metabolic processes, cell signaling, and related physiological phenomena. They can be isolated from many kinds of tissues, including those in the skin, blood, kidneys, bone marrow, musculoskeletal system, and cardiovascular system.   In their utility for various research applications, human primary cells share much in common with immortalized cell lines. Unlike cell lines, however, primary cells have a limited lifespan. Yet human primary cells...