Mouse Primary Cells
With the advancement of cell culture technology, the application of primary cells isolation and culture has gradually become common after the 2000s. Compared with the immortalized cell line, which can be cultured in vitro for a long time, the primary cells are closer to the actual physiological process in vivo , especially in the project based on mouse models. Therefore, mouse primary cells became a more general choice for researchers in project design based on mouse models. W hat are Primary Cell s ? Primary cells are freshly obtained from a multicellular organism and cultured in-vitro cells. Unlike immortalized cell lines, primary cells cannot be subcultured indefinitely due to senescent-phenotype-induced irreversible cell cycle arrest. The survival and growth of primary cells have higher requirements for culture substrates and nutrient conditions so that their applications are not popularized until the 21st century. Primary cells present se...