AcceGen

Introduction What are Stem Cells? Stem cells refer to undifferentiated or partially differentiated cells in multicellular organisms. Stem cells can replicate indefinitely and differentiate into different types of cells with various functions [1]. The research of stem cells originated in the 1960s, Ernest McCulloch from the University of Toronto and James Till from the Ontario Cancer Institute identified the key properties of stem cells [2, 3]. According to the classic definition of stem cells, it needs to have the following two core properties, namely self-renewal capacity and differentiation potency. Self-renewal capacity can maintain the stem cells population, which depends on the asymmetric cell division mechanism and the protective effects of telomerase  (Figure.1)  [4, 5]. Potency refers to the differentiation potential of stem cells. Based on potency, stem cells can be divided into totipotent, pluripotent, multipotent, oligopotent, and unipotent stem cells [6]. There are...

Brief Introduction  to PBMCs Peripheral Blood Mononuclear Cells (PBMC), also called Human Mononuclear Cells from Peripheral Blood (HMNC-PB), are cells with a single, round nucleus that are collected from peripheral or circulating blood. These blood cells are a key component in the immune system to fight infections and disease, adapting to invaders. PBMCs respond selectively to the immune system and are a key component of human immunity. PBMCs are a mixed population of monocytes composed primarily of lymphocytes, including T cells, B cells, and natural killer (NK) cells.   PMBCs are also a rich source of monocytes, such as macrophages, dendritic cells. Further cell isolations are performed by selecting key protein surface markers such as CD4 +  (T helper cells), CD8 +  (cytotoxic T cells), CD14 +  (monocytes), etc.   PBMCs undergo characteristic changes in various disease states and represent a way to monitor disease progression and understand...